The Role of Metagenomic Next-Generation Sequencing as a Promising Technology for Diagnosing HIV-TB Coinfection

The human immunodeficiency virus (HIV) pandemic has caused a resurgence of tuberculosis (TB), thus increasing morbidity and mortality. Moreover, HIV-TB coinfection leads to difficulties in diagnosis. Sputum smear microscopy, mycobacterial culture GeneXpert MTB/RIF assays are generally endorsed detect Mycobacterium ( M. ) coinfection. However, these methods cannot diagnose TB an accurate timely manner, the rates HIV-associated mortality patients with TB. Hence, considerable need exists for better diagnostic tools Metagenomic next-generation sequencing (mNGS) is novel detection platform widely used assess infectious disease, antimicrobial resistance, microbiome host gene expression. Herein, we summarize advantages mNGS disease diagnostics. We then efficiency different specimens several cases conclude that acceptable method coinfection, although limited research available.